CRISPR in a bacteria?

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In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

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Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

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Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Anushree Basu

Clustered regularly interspaced short palindromic repeats (CRISPR) is not a bacteria but are segments of prokaryotic DNA containing short repetitions of base sequences followed by short segments of "spacer DNA" from previous exposures to a bacterial virus or plasmid. The CRISPR system is a prokaryotic immune system that confers resistance to foreign genetic elements.

Johannes Dick

A little more information on what you are trying to achieve might be helpful... A good starting point for further reading concerning CRISPRCas9 based genome editing in bacteria might be this review:

http://www.cell.com/trends/microbiology/fulltext/S0966-842X%2815%2900019-0