- Recently, I was studying special drugs. I think CRISPR can be used as a drug research program. I have two plans. The first one is to edit on the basis of cas9. The research of cas9 is not very smooth, and it may miss the target in the human body. This will cause cancer. Edit it, let it change genes instead of cutting genes, so that cells can be immune to diseases Virus, this will not damage good cells, but also make good cells immune to the virus, so that the virus infected cells recover or die, very safe. The second scheme is to edit the new cas13 and let it cut off the RNA of the virus. Its targeting stability can be used to solve this problem. I want to know if this method is feasible. I'm working on this project. Who are you interested in? Interested can send me information, also hope to research drug personnel useful, thank you.
Cut-less editing does not by itself solve off-target effects. Off-target editing could be just as mutagenic as off-target cutting.
I like your cas13 plan. Off-target RNA editing certainly sounds less harmful than off-target DNA editing. RNA is short lived after all and would be re-made from good DNA.
Now you're just left with the usual problem of delivering enough of your macromolecular complex into enough cells to have a clinical effect. What are you going to do about that?
In my opinion Cas9 protein can be used to cut and edit the target gene but to target particular gene in a specific cell inside human body is a big challenge. Moreover if Cas9 will be delivered somehow to those cells continuous expression of Cas protein in the cells would ultimately leads to off target effects. so in my opinion it would be difficult to use Cas as a tool to get rid of virus infection.
Cut-less editing does not by itself solve off-target effects. Off-target editing could be just as mutagenic as off-target cutting.
I like your cas13 plan. Off-target RNA editing certainly sounds less harmful than off-target DNA editing. RNA is short lived after all and would be re-made from good DNA.
Now you're just left with the usual problem of delivering enough of your macromolecular complex into enough cells to have a clinical effect. What are you going to do about that?
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