CRISPR (/ˈkrɪspər/) (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. These sequences are derived from DNA fragments of bacteriophages that had previously infected the prokaryote. They are used to detect and destroy DNA from similar bacteriophages during subsequent infections. Hence these sequences play a key role in the antiviral (i.e. anti-phage) defense system of prokaryotes .
CRISPR gene editing is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified. It is based on a simplified version of the bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added in vivo.
Hi there
I think the most close book to your inquire is the following best practical book:
CRISPR Gene Editing, Methods and Protocols, Editors: Luo, Yonglun (Ed.).
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Please refer to the following articles.
1. Doudna J.A. and Charpentier E. 2014. The new frontier of genome engineering with CRISPR-Cas9 . Science 346 (6213): 1258096. DOI: 10.1126/science.1258096
2. Wright AV1, Nuñez JK1, Doudna JA2. 2016. Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. Cell, 164(1-2):29-44. DOI: https://doi.org/10.1016/j.cell.2015.12.035
3. Barrangou, R., Doudna, J. Applications of CRISPR technologies in research and beyond. Nat Biotechnol 34, 933–941 (2016). https://doi.org/10.1038/nbt.3659
This has been applied in different aspects, you can as well find out more about CRISPR/Cas tools in specific area of your research in biochemistry or biotechnology
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