Statistician wanted for collaboration?

Statistician required for collaboration on a research project. The project sought the opinions of medical imaging professionals about providing a preliminary image evaluation to referrers....

01 May 2019 9,048 5 View

How does one compute confidence intervals for the difference between two binomial proportions in SPSS?

I have a relatively small data set of 135 subjects. These are categorized in two dichotomous variable, type of health care and sex. I can use a Fisher's Exact test to determine if sex is...

05 March 2019 3,847 5 View

Schema therapy , Personality disorders , Older adults . What more can we learn from older patients of PD BPD to enhance therapy ?

I am currently in early stages of qualitative research design in the areas of perceptions and exploration of older adults . I am looking at investigating older adults with personality disorders...

19 February 2019 7,117 5 View

Non-parametric tests: PermANOVAs, or Kruskal-Wallis test?

Hello, Because my data has non-normal distributions, I understand I need to run non-parametric tests. My data includes multiple samples from several distant collection sites. Under what...

22 January 2019 5,467 5 View

Funding is a major problem for me. Is it less costly to check for pathogens in ticks via PCR or antigen/antibody match?

I am unclear if antibody tests can be conclusive in a tick or even if it is possible to test. If so, what are the limitations once the ticks have been collected. Limits such as temporal limits,...

08 January 2019 1,642 7 View

What evalues are recommended for BLASTing fungal sequences?

Hello, I'm using the command line to NCBI BLAST fungal DNA sequences (Table-C1, https://www.ncbi.nlm.nih.gov/books/NBK279684/). The default evalue is 10.0, but one paper I'm following used...

17 November 2018 1,031 3 View

Bacteriophage CE6 wanted - can anyone help?

I have been trying to get a sample of bacteriophage CE6 (which expresses T7 polyemerase) for protein expression studies. The lysogenic form of this is the (DE3) you see in genotypes of cells used...

14 November 2018 2,954 3 View

Grouping read IDs by sample names, and sorting samples into separate fasta files?

Dear colleagues, I pooled together dozens of samples into one library, and sequenced them together. This yielded millions of reads, with the sequencer assigning each unique read-IDs. While the...

08 October 2018 5,513 4 View

What are acceptable thresholds when using BLASTn on fungal DNA sequences?

Hello, I'd like to know your opinions on identifying fungal DNA sequences using the BLASTn with regards to Max Score, Total Score, Query Cover, E-value, and Identity match %? What are common...

06 July 2018 9,896 1 View

Similarity thresholds for fungal OTUs?

Hello, I understand there's disagreement regarding the similarity threshold for fungal OTUs, be it 95%, 97%, or 90-99%. In the literature, anyone of these threshold may be cast as adequate or too...

01 June 2018 3,308 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Has anyone used Whatman FTA Plant Saver cards for collection of fungal DNA?

I would like to barcode fungal caps from the field for two main research goals (1) molecular iD of the fungal specimen and (2) to identify any invertebrate DNA within the cap using CO1...

08 February 2021 4,486 1 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

ECG and children with autism ?

Does anyone have experience with collecting ECG data on children with autism? How was your experience? I'm interested in the tolerance levels for kids to have wires attached to their chests. What...

02 February 2021 5,850 3 View

List any intelligent frequently ask questions among poeple regarding special need education and people with special need?

i need any question that is related to the special need education or the people special need (exceptional children)

25 January 2021 7,851 6 View

What aspects of communication skill improves significantly regarding to the Autism Spectrum Disorder using PECS?

Children with ASD need a clear and effective way to communicate to reduce frustration and replace challenging or unacceptable behaviours (Beukelman and Mirenda, 1998; Webb, 2000). Augmentative and...

19 January 2021 1,160 3 View

How to measure/estimate media pH inside the incubator?

I am having an issue with measuring the pH of my media. I use D-MEM/F12 media (without buffer) and try to adjust it to pH = 7.4. Since I incubate it @ 37C / 5% CO2, I added 2.5 g/L of NaHCO3...

18 January 2021 3,816 1 View