Hi everyone!
I'd like to know more about CRISPR and its uses and possibilities and I'm looking for courses in European institutions. Do you know about some place specialized I could go and train?
Thanks!
I'd like to measure enzymatic activity in E. coli and I need to perform a cell extract first. Can anyone share a nice protocol to do it?
01 February 2014 8,473 5 View
I'm running polyacrylamide gels with Cy3-fluorescence labeled DNA oligos. With the small one (26 bp) I obtain a massive background from largest band to shortest, even more intense than the...
12 November 2013 6,548 6 View
10 November 2013 8,174 7 View
I need precast 20% PAGE precast gels for BioRad chamber (15x20 cm aprox.). BioRad does not have a big selection apparently. Does anyone know where can I buy good quality ones? They are for running...
10 November 2013 3,546 2 View
I am looking for some training for new staff that will be starting in a self contained classroom with students with ASD. Most new staff have little to no experience working with students with ASD....
03 August 2024 6,717 3 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another? Which leadership training methodology would be most successful with your...
14 July 2024 173 4 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View