Regarding HPLC: I am having problems with my chromatogram, as the retention times of maltose and sucrose are very close (or the same), so using the chromatogram it is not possible to make this differentiation (The column in use is SCR 101 C).
Robert L. Veazey
You can find this information very quickly with a simple web search. The manufacturer's example for monosaccharides separation on the column you are using does not show sucrose in the chromatogram, so I suspect you will not be able to separate it from maltose and you will need to use a different column. A different column chemistry such as propyl amine is common for carbohydrate separations. If you want to stay with the ligand exchange column type you will need one with a different cation.
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