A few months ago, I submitted my paper to a journal. One of my manuscript's reviewers asked me to quantify the HE and IHC staining of the pancreatic tissue. Unfortunately, now I have no software to quantify that data. So, I humbly request you to suggest me some online tools to quantify that data? Thank you!
You should try Cell Profiler. It is free open source software that will batch process images for you. You can specify what parameters you want to quantify. I used it for quantifying cell density and blood vessel density in the cerebellum. It worked great!
http://www.cellprofiler.org/
If you want to do the same thing manually, I would just use ImageJ. The cell counter tool allows you to easily count cells of various types.
http://imagej.nih.gov/ij/
Good Luck!
Hi Bhesh
You should try ImageJ. It´s very good for that.
Best regards
For small number of samples, where you have to distinguish only in a few types of cells (for example exocrine versus endocrine, such as beta cells), Image J (with an appropriate plug-in for area) will do fine. However, if you are interested in detailed morphometry using information from serial sections of pancreas, use CellProfiler/Analyst as suggested by Leslie. The latter can deal with much larger data set and can almost be automated (if you have the right equipment).
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