Bhesh Raj Sharma

4 Questions 13 Answers 0 Followers

Questions related from Bhesh Raj Sharma

If the western blotting data of a particular protein is not from the same image, how can we labelled them for each sample, such as N, C, S1, S2, etc?

Actually, we measured the expression of a particular protein in the adipose tissue of db/db mice. We loaded the first three samples from the normal group, then three samples from the control...

11 November 2014 2,859 8 View

Could you please recommend me some online software/tools to quantify the HE and IHC staining of the Islets of Langerhans?

A few months ago, I submitted my paper to a journal. One of my manuscript's reviewers asked me to quantify the HE and IHC staining of the pancreatic tissue. Unfortunately, now I have no software...

11 November 2014 4,463 3 View

How could we fix the dose of the herbal extracts (whose active constituents are unknown) in an animal assay?

Before studying the activities of the extracts or compounds in a cell cultured system, we usually prefer different cytotoxicity assays, such as MTT, to fix the safe dose of the extracts or...

08 August 2014 3,787 4 View

What is the apropriate protocol to measure glucose uptake in 3T3-L1 cells and L6 cells using 2-NBDG?

I’ve followed different recent papers to measure glucose uptake in 3T3-L1 cells and L6 cells. The problems I found are (1) that fluorescent values obtained are small and (2) it’s hard to see the...

02 February 2014 2,507 23 View