Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

What is the system of units used in the thermal conductivity by Spitzer and Harm?

23 February 2021 9,253 1 View

How many days do we need to detect adoptively transferred Treg in the tumor site in tumor-bearing recipient mice?

Hello, I've got a plan to isolate mouse splenic Treg (WT and KO of target gene) and inject these CD45.2 Treg into tumor-bearing CD45.1 mice. My question is how many days do I need to wait to see...

11 February 2021 8,238 1 View

Is it possible to antibody labeling with FITC cause antibody disfuction?

Hello, I studied how much bound my test antibodies to the target antigen using FITC labeling to test antibodies. I tested 3 types of antibodies, the elisa binding affinity and cytotoxicity...

07 February 2021 8,335 2 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Is there a surface inactive water-soluble dye?

Hi guys, I'm looking for a water-soluble dye which is not surface inactive in order to observe (water in oil) emulsions; I don't prefer to use oil-soluble dyes. I've used rhodamin123,...

21 January 2021 8,496 2 View

Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How can I build one phylogenetic tree for an arthropod community when I had known their scientific names?

I am working on the phylogenetic analysis for communities of plants and arthropods. A standardized protocol of building phylogenetic tree for plant species list that was widely used in studies....

29 December 2020 181 3 View

Why can I not see any plaques when performing a viral plaque assay with MHV?

28 December 2020 5,515 2 View

What was scientist thinking about Bees communication before Karl von Frisch discovery?

In the 1920s, Karl von Frisch pointed out that bees use special dancing patterns. What scientist or people think about bees communication before its discovery.

22 November 2020 1,426 5 View

What are the main taxa of above ground arthropods in a silvopasture system in the tropics of Latin America?

Beside dung beetles, I would like to learn more about the diversity of arthropods in silvopasture systems where livestock are kept on rotation. Also what about arthropods diversity in similar...

31 October 2020 6,632 1 View

How can I calculate cell survival percentages from a polynomial regression calibration curve?

Dear all, I performed an in vitro luminescence bioassay to determine the effect which an arthropod toxin has on the cell viability (CellTiter Glo) of mouse myoblasts and neuroblasts. After blank...

22 October 2020 2,841 15 View

How to convert Gro files to Pdb using gromacs?

Hello, After I minimized the protein structure I used editconf in gromacs to convert the minimized GRO file to PDB. when I visualized the PDB file produced, it was totally corrupted which...

09 October 2020 547 4 View

Preserving mites in a mixture of ethanol and propylene glycol?

Ethanol and propylene glycol are both used to preserve mites, and each have their drawbacks (i.e., evaporation and specimen distortion). Some people add a small amount of glycerol to vials of...

08 October 2020 9,516 6 View

Thank's a lot again , I was reading about the reckittesiose and I know that the transmission is with a vector which is Tick? is Mosquito can transmit?

Is there any study published and demonstrated which indicates the presence of the agent of rickettsioses? in other arthropods not hematophage.

02 August 2020 5,506 3 View

What is the relationship between Ubuntu//Botho philosophy and Gender?

The African philosophy of Ubuntu is highly praised for its understanding of one's humanity as the capacity to recognise the other. What is the link between Ubuntu and gender? Is Ubuntu gender...

31 July 2020 3,586 5 View