Hello to everybody....how can i get better result of 260/230 in RNA extraction with trizol?

i have RNA that i extracted it with trizol. it has good concentration ( 1010ng/ul) and good 260/280 (1.9) but 260/230 isn't good ( 0.5)....now how can i improve it? can i use again from this RNA...

24 January 2020 4,409 3 View

What is the reasons of conformatonal changes of proteins?

are conformatonal changes of the proteins vital?

29 June 2019 521 2 View

How to read case and data file for transient simulation in fsi simulation?

I run transient fsi simulation with fluent and mechanical transient ansys. In post processing for fluent or CFD just show me till 335 time step but my simulation had 425 time step. I don't know...

12 February 2019 9,077 1 View

Can anyone provide a detailed Congo red staining protocol for detecting amyloid aggregates in neuroblastoma cell culture please?

I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...

20 January 2019 287 3 View

Can we model our dreams ?

01 March 2018 9,530 2 View

Do you have a detailed protocol for dissolving Abeta25-35 in DMSO?

Hello, I have no access to HFIP, and I already tried NH4OH method with little success. The ThT assay resaults did not make sense by this method. And AFM showed the 500 nm spheres were formed. Any...

03 September 2017 4,334 2 View

Does the choice of solvent affect the molar extinction coefficient of a particular peptide?

Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...

29 July 2017 1,278 2 View

How get the output strain E

i included field output request as E for total strain, however while getting the output i'am getting the LE output only which is the "logarithmic strain".Is it because my strains are very large...

21 May 2017 4,543 5 View

For transferring cell lines between laboratories in 2 hours distance should I keep the T-flask vertical or horizontal during communication?

13 February 2017 5,140 6 View

What are the advantages and disadvantages of Disdrometer for determining rain drop size distribution?

What are the advantages and disadvantages of Disdrometer for determining rain drop size distribution? And how about other methods like high-speed imaging or etc.? Which methods do you prefer if...

04 September 2016 1,578 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

What is the critical deficiency limit in soil for Mn in wheat crop ? in terms of DTPA extractable Mn?

critical range for Mn in soils is 2.5-4.5 ppm for all plants as an average, but what is the exact amount of DTPA extractable Mn in soil for wheat crop

04 February 2021 4,008 4 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View