Could you be interested in intracellular recording of beta cells in microdissected islets of Langerhans?

More Juan V. Sanchez-Andres's questions See All

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

Does anyone have access to literature supporting the idea that there are differences in energy demand across different levels of a building?

I'm working on the theoretical framework of my thesis and I can't find literature to support the idea that at different floor heights, there may be differences in energy demand. If someone can...

25 March 2024 4,998 1 View

Is anybody interested in initiatives to curb food waste at the consumption stage?

"Creative and Sustainable Initiatives to Combat Food Waste at the Consumption Stage" This Special Issue on combatting food waste through responsible consumption aims to gather the latest results...

29 February 2024 3,822 6 View

What are those shinny/metalic crystals?

I have recently commenced research involving sulfate-reducing bacteria. For my experiments, I prepared an anoxic freshwater medium supplemented with trace elements, a vitamin mixture, and sodium...

29 January 2024 4,306 2 View

A good anti-GR antibody for western blot in plants?

Hello, I would like to perform a western blot to detect a protein fused to GR (Glucocorticoid Receptor) and expressed from a 35S promoter in transgenic apple plants. I have never used anti-GR...

17 January 2024 7,806 0 View

Fluorescence properties changed due to laser irradiation?

Hi everybody. I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation. For...

16 January 2024 8,821 4 View

How can I lower the viscosity of a water- Carboxymethyl cellulose solution?

I`m trying to prepare a solution of water-cmc with the highest content of CMC possible for further processing, but I wanted to know how I can lower the viscosity so it will be easier to handle but...

04 January 2024 2,062 6 View

How can we enhance histological sectioning techniques to improve the quality of tissue?

The liver sections look fragmented, how could the histological technique be improved to better observe this tissue under the microscope?

06 December 2023 7,388 3 View

Does anyone know how to remove the chromogen from an immunohistochemistry?

During my immunohistochemistry procedure, after applying the chromogen diaminobenzidine, I mistakenly added other reagents—PBS, Triton, avidin, and biotin—resulting in chromogen precipitation and...

30 November 2023 6,702 1 View

What equipment is suitable for delivering electrolytic lesions to mark electrode location?

Hi, I would like to mark the tip location of a NeurNexus probe. I know a good way to easily find the electrode location is to anesthetize the animal, perform an electrolytic lesion, wait a few...

21 October 2023 1,656 1 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

What are the future implications of quantum computing on image processing algorithms?

Image Processing Algorithms, Quantum Computing.

17 July 2024 7,958 2 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

What are the radiometric correction procedures that are applied in Agisoft Metashape?

Dear RG-Community, I have been using Agisoft Metashape for UAV imagery processing for quite a while now. Also a while ago, I stumbled upon the Micasense GitHub repository and saw that individual...

17 July 2024 8,580 2 View

Leslie S Satin

Hi Juan,

Yes I would. We should talk more. Can you email me at my regular address: [email protected]?

Many thanks,

Les

Enrico Cherubini

I retired from SISSA few years ago and I took the direction of the European Brain Res Institute (EBRI) founded in Rome by Rita Levi-Montalcini. I have a very small group there working mainly on the in vivo hippocampus using optogenetic tools.

Best wishes,

Enricp

Zerihun Assefa

Hello Juan,

Thanks a lot for noticing in our work. Your project is potentially interesting for later stages of our work, but not any the moment.