We want to develop the assay to test if certain enzymes could use polysaccharides from mucus as a substrate and we need a simple protocol how to isolate mucus and/or glycosylated proteins from mucus only from mouse small intestine.
In our lab, we investigated ethanol effect on glycoproteins separated from microvillous membrane and mucus. We developed a method for isolation of glycoproteins for analysis. You may get some lead here...