Relatively new to flow. Have learned what I know ad-hoc and would like to learn to be a better practicioner.
The "bible" for flow cytometry is the book "Practical flow cytometry" by Howard Shapiro: http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471411256.html
However, this is a rather lengthy book and probably too detailed for most beginners. If you want a rather compact overview, I would recommend you downloading the free ebook from Tim Bushnell at Expert Cytometry: https://expertcytometry.com/
Lastly, the single best review summarizing most relevant topics of flow cytometry is this here: Chapter Basic Multicolor Flow Cytometry
if you read and understood this chapter, you are good to go.Good luck with your flow cytometry experiments!
The "bible" for flow cytometry is the book "Practical flow cytometry" by Howard Shapiro: http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471411256.html
However, this is a rather lengthy book and probably too detailed for most beginners. If you want a rather compact overview, I would recommend you downloading the free ebook from Tim Bushnell at Expert Cytometry: https://expertcytometry.com/
Lastly, the single best review summarizing most relevant topics of flow cytometry is this here: Chapter Basic Multicolor Flow Cytometry
if you read and understood this chapter, you are good to go.Good luck with your flow cytometry experiments!
Dear Daniel,
in addition to Florian's excellent recommendations, I would like to mention the recently published guidelines for flow cytometry written by a multitude of international experts.
Article Guidelines for the use of flow cytometry and cell sorting in...
Dear Daniel,
in addition to above recommendations, I would like to suggest to you Current protocol in Immunology. a very good book and chapters 5.0 -5.7 gives an elaborate and extensive ideas toward flow cytometry.
Thank you all for these recommendations.
Have a great year and best of results w/ your research.
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