How to preserve endogenous GFP/RFP during IHC?

I have a dual reporter mouse that expresses RFP and GFP under two promoters. I would like to also do IHC on this tissue but the antigen retrieval step (heat) denatures the GFP. Moreover, if I skip...

11 December 2019 403 3 View

Is anyone aware of a complex database of ARM Cortex M processors?

Is anyone aware of an overview database of ARM Cortex M processors for most licensed manufacturers, listing Flash, ROM, clock etc? I'm trying to figure out common specification classes.

16 October 2015 1,522 1 View

Can someone write me how I can decode the exe.stackdump?

I use Cygwin64 linux emulator on Windows 7. After I had run an f.90 code I got the SIGABRT:Process abort signal message. I would like get to know from exe.stackdump file what causes this issue....

10 November 2014 5,865 0 View

Does anybody have experience with cdo installation to UBUNTU 14.04?

I would like to use cdo in UBUNTU 14.04. I built and installed the required libraries (zlib-1.2.8, hdf5-1.8.12,grib_api-1.9.18, jasper-1.900.1, netcdf-4.2.1.1, cdo-1.6.4rc4) by the following...

08 May 2014 410 4 View

Does anybody have experience with cdo installation to UBUNTU 14.04?

I would like to use cdo in UBUNTU 14.04. I built and installed the required libraries (zlib-1.2.8, hdf5-1.8.12,grib_api-1.9.18, jasper-1.900.1, netcdf-4.2.1.1, cdo-1.6.4rc4) by the following...

04 May 2014 7,959 4 View

Must subscript indices either be real positive integers or logicals?

I would like to calculate the standard deviation of cloud droplets diameters which are between 4.24-53.43 micrometers. When I use the std(x) function of Matlab I get the following error...

04 May 2013 6,124 0 View

I used the lognpdf(x, mu, sigma) function in Matlab 7.11.0 (R2010b). The value of x, mu and sigma were both real data.

03 April 2013 1,808 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View