Could someone help me out in recombinant protein purification?

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Why might my GFP tag be dissociating from my plasmid after transfection?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Are protein samples in SDS compatible with ELISA?

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Unknown debris sheets observed in cell culture flasks?

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Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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Bjarte Aarmo Lund

Hard to answer without any details. Which purification-strategy have you used?

IMAC or IEX? How many steps?

Dominique Liger

Contamination or degradation products? The picture of the gel is more than welcome.

Parismita Kalita

Kindly provide the detailed purification procedures you have used here.

Philippe Urban

Hi Sai,

As already stated by others, please specify to us what you are doing with more details.