Basically, I am using the protocols described by Meriluoto and Codd (2005). [Meriluoto, J., Codd, G.A., 2005. Toxic: Cyanobacterial Monitoring and Cyanotoxin Analysis. Åbo Akademi University Press, Åbo, Finland]. This book you can find here: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0CCAQFjAA&url=https%3A%2F%2Fwww.doria.fi%2Fbitstream%2Fhandle%2F10024%2F76837%2Ftoxic.pdf%3Fsequence%3D1&ei=A0BaVcjbF4aeygOhqICgAg&usg=AFQjCNG7p2ki3csioH9ZpWF7kAFal4vXyA&sig2=NxtNR0glC_UO1zh7tpXhKw