Could somebody be willing to provide me with plasmid samples of Pichia Pastoris for a potential collaboration?

Viviana Belloso @Viviana-Belloso

28 September 2020 1 6K Report

If you wish to know more about the project, please contact me via private message.

Michael J. Benedik

You can buy kits for Pichia which include strains and plasmids.

Badges
Science topic

More Viviana Belloso's questions See All

Hi there, do you know a competitive italian company where to buy microphones for Ultrasound Vocalizations detection in rodents?

I want to buy microphones for USVs detection. I have been always using the ones from Avisoft (CM16/CMPA). I'd really appreciate suggestions about a comparable kit from Italian company. Thank you

19 March 2024 7,251 0 View

Is it correct to use human plasma in heparin/sodium tube for Elisa Kit?

We purchased an Elisa Kit for Human Insulin and the manufacturer indicated the use of Heparin/Lithium tubes. Unfortunately, the samples were collected in Heparin/Sodium tubes. Will the results...

27 December 2022 424 3 View

Can i use primers from a part of intron to the contiguous exon to get information about primary transcript (pre-mRNA) of a specific gene by RT-qPCR?

Hi everyone, im interested into knowing the quantity of spliced and unspliced mRNA for a paticular gene. I thought using primers for mature mRNA (exon to exon) and other set of primers to quantify...

27 April 2022 6,950 2 View

Can ultrasound waves of vocalizations travel in plexiglass without much lost?

Hi there, I'm going to perform a behavioural experiment in a closed plexiglass chamber, linked to a ventilation system through a hole (let's think about it as a fume hood). In this experimental...

27 April 2022 6,322 1 View

How do you perform Immunofluorescence staining on thick brain slices (around 400 micrometers)? cut and then stain or whole mount stain and then cut?

Dear collegues, how do you would perform Immunofluorescence staining on thick brain slices (around 400 micrometres) upon acute slices pharmacological manipulation or electrophysiology? -cut and...

10 March 2021 1,652 2 View

The best assay for serum samples for total protein content?

I usually use the Bradford Assay, but have had problems (high values... I used 1: 100 dilution, Should I dilute it more?). These serum samples are from rats that have consumed CD or HFD, and a...

17 October 2020 652 8 View

What books on anticancer potential of polysaccharides obtained from fungi recommend me to read?

I am investigating the medicinal potential of the polysaccharides present in edible fungi native to my country and that can help prevent or treat some types of cancer.

16 February 2020 8,013 3 View

What type of blanking solution for Nanodrop could I use to measure the DNA quality of samples dissolved in solutions unknown?

I am working with samples of cryopreserved DNA in different years of a Biobank, so when I am going to measure the concentration or purity of DNA, a big problem arises. This is that there are no...

13 May 2019 3,883 5 View

Could anyone help me to know why dinydrated zinc acetate could not be soluble?

It is suposed to be soluble at 430g/L in water and in ethanol. But that does not happen with the one I received. I guess 1. It is not 100% 2. It is adulterated but with what?

26 September 2017 2,510 2 View

Forest values analysis: free listing and ranking. Is it possible to analyze them together?

Hi everyone, I am trying to determine an "order" of collected information about forest values from informants of different ethnic groups: 1) Using the free-listing method I collected the first...

24 August 2016 4,408 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?

03 August 2024 607 2 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View