Mammalian cell expression systems are utilized to produce special proteins that cannot be correctly folded or modified in Escherichia coli. However, the cultivation of mammalian cell is much more difficult than that of E. coli.
Protozoans, such as Paramecium caudatum and Amoeba, are microorganisms which are easy to cultivate. Bestides, these organisms are also belongs to animal. Therefore, is it possible to develop protozoans as an easy - to - cultivate expression system with the ability to efficiently and correctly express heterologous proteins ?