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Hi there! I'm very new to r and trying to run a multi-level meta analysis using pre-calculated effect sizes. I've attached the datafile below. The script I used as a first step to fit the model...

12 January 2021 8,214 2 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

Can hypoxic air (1% O2, 5% CO2, 94% N2) buyed from the market?

I need hypoxic air (1% O2, 5% CO2, 94% N2) for hypoxia experiments, can I get/buy some hypoxic air from the market?

14 July 2020 5,471 2 View

How to improve the thorn resistance of textiles?

Unlike body armor, the body armor faces a smaller area of knife tip. Although the energy is small, some body armor does not prevent the knife from penetrating.Moreover, if the use of composite...

24 November 2019 928 4 View

Theoretical max-min of Gibbs free energy?

Hello all, Does anyone know if there is a theoretical maximum and minimum for the Gibbs free energy? In other words, under constant physiological conditions, can one calculate the possible...

04 July 2019 8,773 5 View

Optical and radiative properties of molten salt?

The thermal radiation transfer in molten salt is usually neglected in researches. Does anyone knows the reason ? Are there any research (experimental data) focusing on the absorption coefficient...

30 June 2019 1,243 3 View

What about Multiple comparisons in a linear mixed model in R?

I'm doing the analysis with a linear mixed model.Because the sample size is different, the Scheffe test is used for the multiple comparison of my data. However, I do not know how to write code in...

09 January 2019 2,517 6 View

Apply concentrated pore fluid: This node set is not active in the model?

Hi everyone, I was modelling DFIT (Mini-fracturing) in Abaqus and applied a concentrated pore fluid into my model. However, there is error saying: A concentrated load has been specified on node...

04 December 2018 7,840 2 View

How to simulate the wick structure in a loop heat pipe?

Dear researchers: I am a newbee in the CFD simulation. Now I wish to simulate the wick structure by Fluent and the evaporation process in this region. The wick is saturated with water and being...

18 September 2018 9,281 12 View

Research problem in Agriculture using predictive analytics ?

How predictive analytics can be used in agriculture?

22 June 2018 4,484 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

What are the validation parameters for RAW BLUE macrophages 264.7?

Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...

24 January 2021 589 2 View

Which compounds might cross-react with cortisol in Elisa kit?

Hi, I'm analyzing cortisol in post smolt salmon with two elisa kits. The first is made for human saliva (Fairly cheap) and the second one for fish (three times the price). I was hoping to get...

21 January 2021 6,874 1 View

Is it possible to detect oxPAPC in the serum?

I would like to know if there is the possibility to detect oxidized phospholipids (oxPAPC) in the serum of mice. Is there an ELISA kit available?

10 January 2021 3,803 2 View