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fetal bovine serum (FBS) is a common supplement for cell culture. my questions: 1. does the FBS have some hormone residues (steroid)? 2. the best method to measure the steroid in medium?
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after release from glucocorticoid (GC) response element, dose GC dissociate from GC and glucocorticoid receptor (GR) complex? Or the GC and GR complex are reused to binding the GC response element?
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