10 April 2015 4 834 Report

I am interested in chemical, biological , physical removal methods. Techniques involving recycling water in situ using existing infrastructure/systems is welcome.

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Do mature (virgin) forests take up carbon, if there is no net increase in biomass?

I seem able to get any answer I please just by searching a little more. Where is the truth? Concerning untouched Amazon rain forest, when people measure carbon flux, do they include the carbon...

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If one wants to link together power sources all across North America, would it be better to use DC transmission lines, or gas pipelines, say with H2?

Intermittent renewable energy sources become more efficient and reliable as the number and diversity of sources is increased. But electrical transmission over distances greater than 2000 miles...

20 October 2020 731 5 View

Using antimicotics such as Nystatin or amphotericin B in mouse neuronal cultures?

Hi all, I just started to prep mouse cortical neutrons for E16.5 and in my second prep I had a massive fungal contamination. Since these preps are quite precious, I wonder if I could add an...

15 September 2020 645 1 View

Is it possible to add a selection agent in using pCAGGS for transfection of HEK-293T cells?

I will try to express proteins in HEK-293T cells. I had previous experience in another cell line where I used co-transfection for selecting transfected cells. I had observed lower levels of...

16 May 2020 2,857 1 View

Does anyone have a general Hydrophobic Interaction Chromatography Protocol?

I have a sample suspended in 0.34 M NaCl after using ion exchange chromatography. I would like to perform hydrophobic interaction chromatography (HIC). Is it okay that I use greater salt...

27 August 2019 8,417 1 View

What is the difference between the columns Hi Prep 26/60 S-100 HR and Hi Prep 26/60 S-200 HR?

I am trying to have a better resolution during purification. Would running my sample using S-100 HR give a better result from an S-200 HR run? Also, other than the fractionation range, what is...

24 July 2019 4,378 3 View

Exemplary models of elementary school speaking-listening skills group instruction-assessment: Successful school sites and teachers?

School districts make public claims and governmental contracts regarding essential English Language Arts Speaking skills, however transparency and accountability is lacking about implementation....

22 January 2019 2,420 3 View

For EV development, what is a good number for battery energy density?

For future EV design, I have tentatively chosen 0.157 kWh per kg. for energy density of the battery pack. Am I aiming too low for a vehicle likely to reach production in five years? Or, is my...

27 December 2018 2,368 1 View

Why the pH of xanthan gum in aqueous solution is 7 (neutral) when it has glucuronic and pyruvic acids in its chemical structure?

I find it pretty confusing since xanthan gum has both glucuronic and pyruvic acids in its chemical structure, it should release H+ ions upon solvation in an aqueous solution and make it acidic.

18 January 2018 9,190 2 View

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