Could anyone please tell me that whether there is a necessity to add 50x oligos with bsa1 restriction sites for digesting a vector with Bsa1 enzyme?

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Can anyone suggest some leading universities worldwide researching on nanotechnology for postdoc?

My area of research is synthesis of metal nanoparticles and their applications focusing on protein extraction from complex biological mixtures using SDS page gel electrophoresis technique. Please...

09 February 2021 2,941 3 View

How we reduce the Dyes in Botanicals like cotton pods(dry)?

For Example: If we take the botanical (Amra Pods) and we are using the dyes to give color to Amra pods . Here I take Acrylic Red(Dye). Im using 100% dye to amra pods to get it colored. Is there...

31 January 2021 1,079 1 View

List of scopus/ DOAJ indexed medical education journals?

suggest some indexed journals with low publication charges that publish medical education related research projects

22 December 2020 6,909 3 View

Stagetips for desalting small molecules (not peptides)?

I work with derivatized neurotransmitter molecules. I was using C-18 SPE cartridges previously for desalting them before LC-MS, but now I would like to adopt in-house made stagetips. These are a...

22 September 2020 4,854 6 View

What are some some helpful online sources to learn statistics, right from the basic concepts?

experiment design, data interpretation, research article writing

21 September 2020 7,723 6 View

Does the protein which serve as adjuvant can be use for vaccine candidate?

I have identified certain surface proteins which are showing both B and T cell responce and also use as adjuvant in vaccine formulation. that means we dont need any other adjuvant to induce...

14 September 2020 1,547 6 View

Dear All. Can normal RNA extraction kit be used for RNA extraction from cell-free medium like culture supernatant, VTM, plasma, etc.?

I am asking specifically for RNA extraction for VTM containing COVID-19 SARS-CoV-2 virus particles. Will the extraction kit make difference in total or specific (viral) RNA yield? There are many...

16 August 2020 8,385 2 View

What is the mechanism of action of HCQ+Azi against covid 19 infection?

how H CQ+Azi acts against covid 19?

31 March 2020 4,043 9 View

How kabasura kudineer helps in covid 19 infection?

It is said that kabasura kudineer a product of Indian traditional medicine siddha/ayurveda has some beneficial effects against covid 19 infection. I would like to know more about this.

31 March 2020 1,997 21 View

What happens if I keep on heating an NIR dye?

After a certain threshold temperature, certainly the dye will degrade but before that what kind of changes (in color, structure or other chemical properties)take place ?

31 December 2019 6,407 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View