Dear Rajaneesh Karimpurath Gopinath, 

The following publication may be helpful:

Fly (Austin). 2013 Jul 1; 7(3): 193–203.

Published online 2013 Jul 11. doi: 10.4161/fly.25438

PMCID: PMC4049853

A method for reversible drug delivery to internal tissues of Drosophila embryos

Victoria K Schulman, 1 , 2 Eric S Folker, 1 and Mary K Baylie

Assay design

We aimed to develop a method of delivering drugs to internal tissues of late-stage Drosophila embryos to better elucidate cellular mechanisms of development in vivo. To develop this protocol, we targeted the microtubule cytoskeleton and assessed the effects on microtubules in muscles. Muscle is a well-characterized, mesodermally-derived tissue that undergoes several distinct cellular and morphological changes late in embryogenesis. Thus, it is an ideal tissue in which to evaluate the delivery of drugs to older embryos. Microtubules are an ideal target for our assay as they are abundant, easily imaged, and a common pharmacological target. To specifically target microtubules, we used Nocodazole, an inhibitor of microtubule polymerization, to reduce the number of microtubules present. The effectiveness of drug delivery was then assessed by fluorescence imaging of both microtubules and growing microtubule plus-ends in fixed and live embryos, respectively.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049853/

Hoping this will be helpful,

Rafik

Thank you very much for this reference. But, I am actually looking for a protocol for the nocodazole treatment and visualization of Drosophila larvae, not embryos. Please forward me the same if you come across it.