im trying to enriching methanogenic bacteria from wastewater sludge before i isolating and identificating the bacteria.
i have found some litterature that said the methanogenic bacteria from the sludge could be enriched by using acetic acid as shortcut to enriching the sludge, as we know that acetic acid was main substrate for methanogenic bacteria. but in my litterature it doesn't said about concetration or molarity or the volume of acetic acid.
so, anyone have experience enriching the sludge using acetic acid?i wanted to know acetic acid concentration/molarity that used. or anyone have a paper about adding acetic acid as sludge enrichment?
thanks before
Regards,
The authors in the attached article has done research on methongenic bacteria for textile waste. They have use acetic acid as part of their enrichment. It is not exactly the same system that you are interested in, but it will give you a start.
Thank very much you Maam, it's really help me althought it's not exactly what i wanted atleast it give me some clue
regards,
Hi youssef,
Thank you very much for your attachment, it help me a lot
Regards,
Hi!
What is important with methanogenic biomass, is the load of substrate with biomass concentration (seed). For BMP activity measurement a ratio of 0,2-0,5 g COD substrate /g VS biomass is commonly accepted.
So for a solution of 150g/L of sodium acetate we use a ratio of 2% (v/v) of this solution with anaerobic biomass. The pH need to be between 6,5-8.0.
PS. There are also hydrogenotrophic bacteria that generate CH4 with H2 and CO2.
Yann
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