hi

use vital staining with neobar lam  

Stains used in this issue are

1-Trypan blue stain.

2- Erythrocin B stain.

Dear Patricia Veras,

Pleae try to use "Trypan blue". Trypan blue is a generally stain for select dead cell or living cells. Live cells are not colored, but if cell dead will turn to blue color.

Regards,

T.Pengsakul

Thanks

Patricia, I think propidium iodide should work.

Renato

Renato did you try for parasites calcein that becomes fluorescent inside live cells?

Eosin Nigrocin stain differentiates live and dead spermatozoa invitro. you may please try

Trypan blue stain

you can try non-toxic stain - Alamar Blue 

3-[4, 5-dimethyl-2- thiazolyl]-2, 5-diphenyl–2H tetrazolium bromide (MTT),  this chemical is very useful for lympho proliferative assays. You can see more about it in  an artiicle titled Chemotherapy of Rhinosporidiosis: a Review by S N Arseculeratne

                                     good luck

Ok Thanks

I would recommend Propidium iodide to identify dead cells, too.

I used 1 ug/ml propidium iodide and FACS.  It worked pretty well for L. infantum.  There is no reason why not working for L. amanzonensis.  Give it a try. Good luck.

Trypan blue solution (0.2%) in saline solution containing 0.01% sodium aside, works well and after 2 minutes, you can check the Leishmania promastigotes, we have used it with different Leishmania species.

I tried to use trypan blue (0.4 % in normal saline) to differentiate dead and live L.major, but the dead leishmania that was circle didn't absorb the dye, then it wasn't work perfectly. Had anybody the common experience. thank you for your comments