Hello Francisco,
I think 70 % Iso-propyl alcohol (IPA) is the best disinfectant for destroying, bacteria, fungi, its spores and viruses. I am working with cancer cells, and i am using 70% IPA and i didn't face any contamination problem; so, i recommend you to use it.
All well wishes for your endeavor.
Hello Francisco:
we have very good experience with disinfectant PERSTERIL. It is solution of peracetic acid and it works very efficiently. Another avdentage is thet it is spontaneously degraded in very short time.
Please, See the folowing link:
http://www.eurosarm.cz/web/structure/persteril-kyselina-peroctova-44.html?lang=en
Best regards
Vit
Hi Francisco,
In part this is dependent on the surface material, but I use a two step disinfection. There is a good discussion of various disinfection methods at:
https://www.cdc.gov/hicpac/Disinfection_Sterilization/6_0disinfection.html
I personally use freshly prepared 1:10 dilution (less then one week old) of household bleach ( sodium hypochlorite final concentration of 0.5%) and let it sit on the surface for 10 minutes. I then use a dry paper towel to pick up any remaining liquid and follow up by wiping the surface down with 70% isopropanol. Some surfaces will be damaged if you leave the bleach solution on for too long.
I hope this was helpful,
Frank
Maybe do it step by step. First, clean your desk regurally with clean rags will removed 90% contaminants. Then 75% ethanol works fine with most microbes. Finally, RNase free reagents will deactivate most enzymes.
IPA is sufficient. Assure wipe disinfection. Don´t spray. Keep all windows closed during work (spores)
In our laboratory, we use a combination of 3 reagents
1) 10% bleach
2)70% alcohol
3) distilled water.
You do them one at a time, and wait each one to dry out.
This practice should work for all lab counters and biosafety cabinet.
But keep in mind this is just a partial part during disinfection. For a complete disinfection, also it will matter where you put the contaminant materials and how you will dispose it from the lab. In our place, we have a commercial company that comes to pick all waste.
1.bleach can be used for virus disinfectant in discard jars but using for surface cleaning is not good as it does not gives ample time for the bleach and organism interaction for deactivation.moreover irritant smell is also a problem.
2.for any lab or clean lab 70 to 80% alcohol or isopropyl alcohol may be used before and after desk work in labs.
3. for desk or lab platform with virus to fungi & spores a wipe with quarternary ammonium compound like microgen D-125 in appropriate concentration is very effective but before that if the desk has any organic dust or material it should be cleaned with proper detergent .
4.a product of Schulke terralin is also effective in conc of 0.5 %.With a damp cloth, wipe down medical devices, floors and other wipeable surfaces. Always aim for complete surface coverage (e. g. 40 ml/m2). Cl
Bleach, but you need to leave some time for it to work before wiping away. I don't think alcohols, that are regularly used in DNA extraction, will have any effect on DNA, but they will kill live microorganisms (assuming it is 70-80% alcohol); the benefit of this is microorganisms on your bench will die and not produce more DNA. So it depends on if it is more important to get rid of DNA or just viable bacteria.
Well, also if anyone would have the financial resources to improve their labs to avoid microbial contamination can use or set the lab with :
"Negative room pressure"
Well, from experience in the food industry, and working in clean rooms. Ozone dissolved in water. Hydrogen peroxide. And of course quaternary ammonium compounds. And there are mechanical consideration such as negative air press to blow everything out of the lab, and UV lights in the right places. And of course not bring things in by using oversuits (Tyvek suit), masks,, gloves and booties (PPE) And depending what you are doing, following aseptic technique is important, like opening sterile packages and using the inside of the package as a sterile field. Of course physically isolating the room would help. https://www.cdc.gov/hicpac/Disinfection_Sterilization/9_0PceticAcidHydroPoxide.html
Hi Francisco,
Cleaning and sanitizing with regular unscented 5%—6% household bleach is recommended. See link from CDC: https://www.cdc.gov/disasters/bleach.html
Some things kills everything.
Chlorine (i.e bleach) and UVC
Some disinfectants kill nearly everything, chlorosan, BZC,
and alcohol (which does not kill everything either).
Summary:
Use bleach on surfaces and UVC if you can afford it (and follow safety guidelines)
On hands, the best thing is to use soap and warm water, with a nail brush, using disposable towel at the end.
As per WHO recommendation sodium hypochlorite 0.5% ( 5000 ppm) can be used for surface disinfectant , and its recommended for hospital surface sanitization.
There is no best Disinfectant. Try as much as possible to manage your samples.
Ethanol, Sterilium combination. (Also formaldehyde would help but better use gloves please)
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