Cell Culture Protocol for 67NR and 4T07 cells?

Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...

02 February 2021 2,590 3 View

How can I calculate Standard Deviation using DataWarrior?

I am analyzing about 500 results in DataWarrior and I need to calculate the mean value and the SD. I managed to calculate the mean value using excel, but it I couln't find a way to simply...

25 October 2020 9,318 3 View

Computer vision / machine learning techniques for "blob" tracking and prediction?

My research involves tracking a dye plume in the coastal zone using computer vision techniques, and potentially making predictions using machine learning. If anyone knows any resources that come...

15 April 2020 6,507 2 View

Escalating dose regimen with osmotic mini pump?

Hi everyone, I wonder if someone has ever used osmotic mini-pump for an escalating dose regimen. I read that some iPRECIO osmotic mini-pump are programmable and therefore can be used for infusion...

03 April 2020 9,009 3 View

Is there anyone that have a trasfection protocol optimized for suspension cell culture?

We work with WIL2 cell line and want to trasfect mirna mimic and inhibitor. We tried first with lipofectamin and than electroporation (using amaxa nucleofector, U-001 program), none of that works....

27 March 2017 4,176 4 View

IWP-2 inhibitor

16 February 2016 1,730 4 View

Is there any solvent to solve SEBS blend?

I need to know the amount of oil present as plasticizer, so I would like to know il i can dissolve SEBS in one solvent and then recrystallyze it in another solvent.

30 September 2015 9,013 2 View

Could you help me with a Capitellidae identification?

Hi, I'm working with samples of the Portuguese Continental Shelf; I found few specimens of Capitellidae with 11 thoracic segments, in which the first is achaetigerous and the other 10 with only...

19 July 2015 1,087 4 View

Is anyone working with PK15 cell line free form circovirus?

I would like to find PK15 cell line free of Circovirus to attempt PCV2 isolation.

06 May 2015 6,617 2 View

Does any body explain why there is a superheater stage in the organic rankine cycles (ORC)?

I am interested in ORC-system. If I look to the hp-diagrams of the main working fluid they do not need a superheating stage, but in reality I saw that in many ORC the fluid is also superheated....

18 February 2015 10,018 3 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

What is the possible treatment for Motile Aeromonas Septicemia in fish?

According to Mahon & Lehman (2019), Aeromonas are distributed in a wide range of marine and freshwater environments worldwide. One way of getting infected by Aeromonas is through the ingestion...

18 October 2020 5,564 2 View

How to illustrate the occupations of different groups according to environmental gradients?

Hi! I have been working with cave invertebrates from semi-arid regions, and have observed some interesting responses from the community to variables at the local and regional level. I know which...

28 September 2020 1,592 7 View

Can you send me bigheaded carp occurrence data from European water bodies?

I'm looking for bigheaded carp (silver carp Hypophthalmichthys molitrix and bighead carp H. nobilis) occurrence data from European waters. Faunistic records are mainly published in national/ local...

24 September 2020 4,177 1 View

How successful use of the DPSIR framework to study the sustainability of wetland ecosystems?

The developed countries have mostly used different approaches, frameworks to achieve the targets of SDGs (Edward, et al., 2007) while restoring the freshwater ecosystems in more productive aspect...

16 September 2020 1,795 7 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

At what stage of development do animals develop sentient?

I'm trying to find out in the development between being an embryo and an adult animal does the animal become phenomenally conscious? I'm especially looking for recommendations for articles on the...

30 August 2020 3,815 3 View

Are there any lists of insect and other invertebrate taxa described from Bogor (Buitenzorg), Indonesia?

In late 19th and early 20th centuries, many species of ants (Insecta: Hymenoptera: Formicidae) had been described from Bogor (Buitenzorg), Indonesia. I want to know any other insect and other...

26 August 2020 657 4 View

No title

Despite the high CO2 of the Paleozoic atmosphere, when CO2 levels were greater than 4000 ppmv (parts per million by volume), scientific evidence indicates a period of glaciation around 445 million...

19 August 2020 6,452 3 View

Does any one know the most abundant invertebrates (shredders) in the Sunderban Mangrove, India?

Any information about the Sunderban Mangrove Invertertebrates would be appreciated

03 July 2020 212 6 View