Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Can cell lines have different DNA content?

I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...

03 February 2021 3,587 2 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

11 January 2021 9,500 2 View

Was there any pesticide disasters other than Bhopal and Kasaragood tragedy around the globe?

Pesticides become prime compo in agricultural activities to meet the growing demand of the human population. But indiscriminate usage and mishandling led to two disasters in India such as Bhopal...

11 January 2021 3,790 7 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

Are the mice expected to develop obvious airway disease after HDM? Will they have signs of respiratory distress at any time during the study?

I am developing model of asthma in mice using HDM. I was asked this question in order to submit the a protocol for developing a disease in animals. Searching in the literature, I can not find any...

30 December 2020 6,455 3 View

Hi, How we can sanitize or prevent fungal growth outside the bags in the high humidity of the chamber?

Hi, How we can sanitize or prevent fungal growth outside the bags in the high humidity of the chamber?

28 December 2020 2,486 2 View

Santolina species, wax moth and bees?

Expect a savage answer and being reported to ResearchGate Admin if your answer clearly shows you did not read the whole question and you are scamming the points system by answering with an...

12 December 2020 4,413 7 View

How we can isolate" distinguish" between Tyrophagus putrescentiae and T. entomophus ?

If I want to isolate Tyrophagus entomophagus by selective media, as I know they can grow on molds of stored grains,fruits, cheese. Or even in beehives debris. But how can I got only one species...

30 November 2020 9,087 3 View

Can we DNA barcode dry Museum insect specimens?

I am an student of entomological field, I am interested to work on DNA barconding of beetles, I have some fresh collection and some Museum specimens as well. I am not much clear that whether...

30 November 2020 7,248 14 View

What is the optimum Wolbachia population that can significantly affect dengue cases in densely populated provinces in the country?

I am working on a paper that looks into the dynamics of the spread of Wolbachia and its potential impact on dengue transmission, particularly in the Philippines.

29 October 2020 6,723 3 View