Can cell lines have different DNA content?

I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...

03 February 2021 3,587 2 View

Could you recommend a Journal to publish a literature review about the relationship between globalization and ultranationalism in Europe?

Currently I am finishing the literature review about the relationship between globalization and the resurgence of ultranationalism parties in Western Europe. This is the first part of my Doctoral...

02 October 2020 9,773 5 View

Can I incubate two secondary antibodies at the same time for western blot?

I want to know if I can use a goat-anti mouse IgM-HRP and a goat-anti mouse IgG-HRP to treat a western blot membrane at the same time. Thank you

26 September 2020 1,332 6 View

What metaphors are explaining the COVID-19 pandemic and how do they impact on the approach that is being considered from public health?

Media discourses around the coronavirus pandemic tend towards metaphorical expressions such as the war against an invisible enemy, of the ecosystem balance so that the Earth returns to its...

31 March 2020 3,098 14 View

State with plural ethical background?

Let A be a person with a consequentialist ethics and B with a deontological ethics. Is it possible that A and B agree on the structure of the State? If so, how would they reach an agreement?

02 December 2019 5,019 6 View

How can I insert initial imperfections on a non-linear analysis from a subset displacement summation of previously elastic buckling analysis on ANSYS?

Hi Everyone, I am working on the strength capacity of clamped-clamped cold formed steel columns with lipped channel sections on ANSYS. For this case I perform a non-linear analysis (ARCLEN), and...

14 May 2019 8,058 12 View

How can I garantee that the CO2 coming out from my biorreactor won't be dangerous in a closed laboratory?

I need to sparge CO2 for my biorreactor, but I'm afraid the excess CO2 coming out from it will build up inside the laboratory. Is there any solution to this problem that doesn't involve expensive...

19 July 2018 9,316 5 View

Could viruses be the creators and basic modifiers of life being a type of bridge between abiotic elements and living organisms?

I mean, viruses are not "live organism" because they need cells to can do any action according with current biology, but, humans are not nothing and cannot live without plants and abiotic...

13 April 2018 5,289 3 View

How can I design a pair of primers with a specific restriction site for an enzyme?

01 June 2016 6,153 6 View

What experiences have with Flipped classroom and improve learning?

Flipped classroom and the impact on learning. There are different experiences that are published about flipped classeoom and diverse found. The improvement of the learning process is important...

22 February 2016 8,771 8 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View