For fractionation of the lignocellulosic biomass, I need to perform quantification of the cellulose and hemicellulose content. For this purpose, I have performed acid digestion of the sample (72% H2SO4), and filtered the samples. Then prepared the DNS follwing this method: (fOR 100ml)
3, 5 DNS 0.63g, Phenol 0.5g, NaOH 0.5g, Na-tartrate 18g. Dissolved in 100ml water and heated for 5 mins at 70 C.
Now, for 96-well plate assay, I need help that how much DNS should be put in the microplate with the filtered digested sample?
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