What is the cause of a smear from direct DNA amplification from human buccal swab samples ?

Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...

07 February 2021 891 3 View

How to edit the coloring in Geneland output?

As the original published paper of Geneland (back in 2005) and most of published papers suggested, the diagrams of posterior probabilities of population membership should group the closely related...

31 August 2020 8,073 1 View

Can Niobium tube act as a protective casing for sintering powder alloy sample?

Hi, as a rookie in powder metallurgy field, I've heard that for sintering CP Ti Powder at 1200C in tube furnace, niobium tube or molybdenum crucible would be used as protective casing instead of...

12 January 2020 1,501 12 View

How to identify the residues that have interaction from autodock or other docking website results?

28 August 2019 655 2 View

Endogenous treatment effect with two endogenous variables?

Hi, I would like to estimate the impact of formal microcredit and informal microcredit on consumption as C = b1X1 + b2X2 + b3M + b4E + ... + e, where X1, X2 are exogenous variables; M is total...

06 September 2018 8,723 4 View

HEK cells coming off after medium change?

19 September 2017 373 4 View

Country Image and Firm Image?

24 December 2016 7,923 1 View

In logistic regression, is a linear relationship always nonlinear if the predictor variable is not limited?

Hi guys, As I am analyzing my data and testing a nonlinear relationship hypothesis between the variables, an interesting question came to my mind which I don't have enough expertise (or I didn't...

01 November 2016 7,759 4 View

Is there anything wrong to separate two groups according to the vertex under a quadratic logistic regression?

Hi all thank you for your attention I have used logistic regression to test whether a predictor and it's squared form would predict a binary outcome. Now that both variables are significant and...

18 October 2016 7,565 2 View

Do I need special stains to make diagnosis of NAFLD/NASH?

My goal is to establish diagnosis of NAFLD/NASH and follow-up disease progression. I obtained some H&E sections of needle biopsy from patients and mouse liver tissues. Would H&E sections...

08 August 2016 366 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to determine solutionizing time for cast Al alloys?

For wrought alloys soaking time at solutionizing temperature is determined by thickness .Do we have such standards for cast Al alloys

23 February 2021 8,839 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

Which thermal conductivity test recommended for Ag-Cu nanofluid?

I have prepared Ag-Cu alloy with polymer coating upon it.... how can I measure thermal conductivity of it ?

22 February 2021 5,915 3 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View