Chemically, is there a possibility the polypropylene film has six radicals or six Oxygen atoms?

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Why is my TE-eluted cloned plasmid (isolated via miniprep) not freezing at -20 degree celsius? Is it normal?

I isolated my cloned plasmid from TOP10 cells using invitrogen plasmid miniprep kit and eluted it in TE buffer. But I noticed that contrary to my plasmid aliquot, it does not freeze at -20 degree...

19 June 2024 1,612 8 View

Can PVA be used as an additive in Aluminum Powder for achieving higher Sinter density?

I am an undergrad student and I need to suggest optimum amount of PVA to be added in Aluminum powder for achieving improved sinter density. Your guidance will mean a lot to me. Thanking in...

25 April 2024 4,892 0 View

Methylation, the best selection to get the real result when is reading the percentage of methylation, a colleague could help me with this question?

I have a important question to analyze the result of methylation percentage, but when we analyze the methylation result we focus in one DNA strand out of the two strands it has, however, we want...

19 April 2024 6,248 3 View

How can I get more cloud storage space on my devices, knowing that I have a university email, but my university only gives 100GB?

I don’t have enough space.

27 March 2024 9,707 0 View

How can I re-concentrate my protein sample?

I have accidently added ripa in the cytosolic protein. How can I re-concentrate my protein sample? I was fractioning the mammalian nuclear and cytosolic proteins.

18 February 2024 5,073 0 View

How coupling between heat transfer and fluid flow in piezoelectric pump?

Greetings, what are the boundary conditions requested for coupling between the fluid flow and heat transfer when designing a piezoelectric pump(piezo actuator)? For heat transfer physics, I mean...

09 February 2024 6,374 0 View

Are there any EPA/WHO trace elements standards in sediment available for Cd, Cu, Fe, Ni, Mn, Pb, Co, Cr, Zn, As?

Any links or table will be appreciable

08 February 2024 1,163 1 View

Encountering Negative Frequencies in Modal Analysis of Reinforced Concrete Structure in Abaqus?

Hello, I am currently conducting a modal analysis on a reinforced concrete structure using Abaqus, and I am encountering an issue where all the natural frequencies calculated are negative. The...

11 January 2024 3,615 4 View

Any idea about the mentioned error below that appears in Comsol multiphysics?

The current RANS turbulence model requires a Reciprocal Wall Distance variable. Make sure that all the study steps solving for the flow variables in this physics interface get their initial values...

08 January 2024 1,697 2 View

What is more accurate way for the gas analaysis. Taking online GC results or through the tedlar bags?

I am doing GC-RGA data for the experiments. When I am doing GC analysis online then there is too much deviation in the results from first-hour run to second-hour run. However, when I am doing the...

02 January 2024 5,799 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

What procedure should I follow for current controlled PPy film electrochemical deposition on ITO?

For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...

06 August 2024 3,176 0 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View