How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View

Could you please share your experience in learning SPSS?

I would like to learn SPSS. Could you please guide me or share your experience if you feel it would help me to learn SPSS? Thank you very much indeed!

08 November 2020 7,357 6 View

How do I compare time domain solution with frequency domain?

I am solving EM wave scattering by PEC cylinder numerically and wanted to compare the scattering width. My result is in the time domain which is evolving with time but the analytical solution is...

11 October 2020 770 3 View

Is it possible to consider intermittency of renewable energy sources by using normally (Gaussian) distributed random numbers ?

Consider wind speed for wind power and solar radiance for solar power. Is it possible to consider intermittency of mentioned sources using normally ( Gaussian ) distributed random numbers in which...

29 January 2020 5,731 3 View

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

12 January 2020 9,036 3 View

How to prepare 1U of lyophilized powder of Bacillus stearothermophilus?

First of all, i have narrow knowledge about antidiabetic assay due to this is my first enzymatic test and i have no one to refer. I have a method but when i tried to google every each of the...

05 January 2020 4,629 3 View

How to compute unknown parameters in an equation from experimental data?

Is there a way to compute some unknown parameters in an equation using curve fitting using experimental data? I am upload a picture which contains the function and three unknown parameters and...

25 December 2019 2,184 7 View

From the Table attached, why Retentivity is decreased for Bi:ZnO 3% and for Bi:ZnO 5%, Coercivity is decreased but Ms is increasing linearly for all ?

Hi all, referring to the table attached, it is seen that when ZnO NWs are doped with Bi at different concentrations, there is an increase in magnetization values initially. But we see by doping...

08 October 2019 3,938 3 View

How to make 500 unit/ml catalase solution using catalase protein from Sigma?

13 August 2019 7,931 3 View

No title

10 March 2019 8,910 11 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View