Hello all,
I am trying to create a graph on excel to express -log10 reduction of treatment groups and CFU spores using the equation -log10(final concentration/initial). However, one of my groups does not have a control to compare to.
How do i overcome this? Analysing data given to me
Thankyou (:
Hello, you can create your own control.
You can find out the CFU of spores by classical decimal dilutions and measuring the OD of suspension if you have correlation between OD and CFU, if no just use approprate nutrient media plates to grow of your spore suspension better in decimal dilutions to more reliable calculation.
Sadly, I think the best advice for you is to drop that particular group.
You can still mention it in text/graph and comment that the control is missing if it's a very important sample. This makes more sense if, for example, all other groups have very similar starting conditions.
The technical solution might be to use the average of all starting conditions (again, assuming they are similar). If they are not similar - there is no good technical alternative
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