Hello Everyone,

I am trying to reproduce the EXACT conditions (at least as close as possible!) for the cytosol in my solutions. From Ca+2 I have found the physiolocal conditions between 100-300nM, and sometimes 1,2 mM interstitial; Mg+2 about 0,7 mM, and pH 7,5. What I am having problems with is the ideal concentration of DTT to reproduce the reducing conditions of the cytosol. Any Ideas?

Thank you

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