hello,
Recently, I got several troubles with my cell culture. I have 8 years of experience with cell culture in my PhD program. I began working in new places and from there~ many problems is coming.
- I used DMEM from Corning , high glucose, include Glutamax, I just add penicillin and FBS 10%. The next day, when I used this media for changing or subculture, cell died, not attach. My cells: MOVAS cells and hVMSC even They grew well before. I just think problem from FBS (out of date=2021) but when I change new FBS, again cell died. No contamination under microscope. So, problem from basal media. (New arrived 2 week ago, just not store in dark place. Everytime, I aliquot 50ml from 500ml bottle into tube and add 10% FBS and 1% p/S. The problem is not coming from the beginner, just after several times using. Do you think because contamination mycoplasma or fungi or because of light?
do you have any trouble like this?
Pham Hoa One potential answer for cell death as you pointed out is mycoplasma contamination. Suggest you test all the reagent you use for cell culture for mycoplasma
I dont think because of mycoplasma.
Cells are still good before I used that bottle. And we also try this plate with 2 kind of DMEM. Another is still working. However, after 2-3 times open this bottle, cells did not attach.
I just think my media degradation because of light or contamination something like fungi have endotoxin (nothing contaminated when checking with microscopy). I have no idea now.
If you're having issues with cell culture media, consider checking for contamination, proper pH balance, expiration dates, and the compatibility of components with your cell line. Ensure that media is stored correctly and follow aseptic techniques during preparation and handling.
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