CD86 immunofluorescence in formalin fixed, paraffin-embedded Mouse Heart?

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

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Issues with Permeabilization during Flow Cytometry?

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01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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How does an antibody have full intact antibody + half antibody?

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28 February 2021 1,577 1 View

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RIA. How to measure?

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Anyone know of a good progesterone antibody?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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25 February 2021 8,404 3 View

Maurice Shen

Hi Renee,

I did a search for you on BenchSci (https://www.benchsc.com) and found several published data that may be of interest to you.

Please see attached for more info.

I hope this helps!

Maurice

Renee R Donahue

Thanks Maurice. I tried that website. Unfortunately they have no publications that I can find that use immunofluorescence to visualize CD86 positive cells in murine cardiac tissue. Many antibodies work in other organs but to date I can not find anything in heart tissue that doesn't require DAB method.