Can you suggest me a way to check Leucine Rich Repeat Kinase 2 (LRRK2) activity?

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Ahmed Soliman

You may consider the detection of phosphorylation of LRRK2 substrates via immunoblotting:

1. Autophosphorylation at S1292

2. T73 Rab10 phosphorylation

3. to a lesser extent, T72 Rab8 phosphorylation

You may also consider Phostag assay for Rab10 phosphorylation, as published by the lab of Alessi doi: 10.1042/BCJ20160557

Good luck

Shivam Bhola

Thank you for your answer