Hello, I've been recently trying to standardize the DCFH method for ovaries.

According to the stablished method, ovaries recently dissected have to be incubated immediatly, embeded in tissue-tek, processed on the cryostat and observed by fluorescent microscopy.

Unfortunately the amount of samples I get in a day take many hours to process and I wanted to ask if it was possible to incubate the ovaries with DCFH and store it in tissue tek in a dark place so I could analyze it later or if I could store at -80°C and then incubate the ovary with the DCFH

I haven't found if this is possible or if HAS to be with fresh samples.