Hi Kaur,  What is the pH of the alginate solution? I think you can try increasing the pH of your algintae solution and warming up the mixture. Other thing is changing the alginate concentration, 0.8%, mixing  with the enzyme and adding the other %. I hope it works, bye.

Hey Enrique, the problem with changing the temperature  and  ph is that we are working with enzymes (sensitive). So i dont think i can change the ph. And if i change the percentage, i may get a final concentration of 1.5% but i wont get an enzyme to alginate ratio of 1:10 (w/w). Thanks anyway :)

Our experience with calcium alginate beads is connected to covering small size plant explants. We use 2,5% solution of sodium alginate. Than it is complexed by a calcium chloride solution (1,1%). All solutions are autoclaved at 115°C (20 min). The alginate dissolves during warming.

Hi,

Why don't you try mixing the dry powders of the protein and the alginate together before adding PBS/DMEM/whatever you use to reconstitute the alginate? Reconstitute this alginate+protein powder mixture overnight with a magnetic stirrer. Should work.

Alginate can form lumps, however you have to leave more time stirring to dissolve completely. We use warm water to dissolve it (you can also sterilize but loses some of its viscosity on cooling) when the lumps are present, we filter with stainless steel mesh of 74 microns.

The protein should be dissolved completely before mixing with the alginate. It is added according to the proportions as if dry.

Hi, Dear

We have this problems now. our beads are very viscous with different sodium alginate (1-5%) and CaCl2 (2-5%).