What statistical analysis would I need for a questionnaire?

Let me first start by saying I'm dyslexic, two universities were unable to teach me research methods and statistics in a way that worked with my dyslexic limitations, and I've continuingly failed...

08 July 2020 8,712 6 View

I'm looking funding and grants for a medical study in deep brain TMS in Vancouver Canada - anybody have any suggestions about who to apply to?

Deep brain TMS study with paired PET scans for individualized targeted treatment based on individual brain functioning reads before and after treatment of deep TMS in identified areas in the PET...

09 March 2020 6,612 3 View

Can a cell membrane be overloaded with too many particles attempting to enter?

When more than one biomolecules attempt to enter into a cell membrane at once (in a large quantity) does it impede the transfection process?

23 July 2019 4,149 2 View

[solved] Can you explain a +44 mass difference in peptide synthesis?

Dear experts, after peptide synthesis (standard Fmoc chemistry, no acetylation between the couplings), I have a significant peak next to my main product for analytical reverse phase HPLC (see...

20 December 2018 782 7 View

Dear, could anyone point me to a good multivariate statistical analysis book?

I want to improve my knowledge in this area. I would like indications of good books that you have studied.

22 July 2018 4,867 10 View

What do you think of the Comet Assay?

Dear Investigator, The comet assay has been in existence for many years, but the general protocol largely remains unchanged, despite it requiring numerous, time-consuming steps. We in the...

12 October 2017 911 3 View

How to stop DAB precipitation during IHC

22 June 2017 2,939 3 View

HPLC-ESI MS analysis of Coenzyme A, Malonyl-CoA, and ATP?

Hi all, we run our HPLC (Agilent 1100 series) usually with C18 or C8 columns, using acetonitrile/water gradient with 0.1 % HCOOH. We have a DAD detector and can attach the HPLC outlet directly to...

07 March 2017 2,150 3 View

How to determine oxygen amount / composition of a gas sample?

Hi, we recently had problems with some syntheses where we use nitrogen flow to remove a byproduct (tBu-SH) from a reduction reaction from a reaction mixture. The educt is a StBu-protected peptide...

17 January 2016 2,441 4 View

Which strategies exist to obtain high molecular weight polyesters?

Which strategies are known in literature to obtain high molecular weight polyesters? I am trying at the moment to react a semialiphatic alcohol at high temperatures with a aliphatic acid, using a...

13 January 2016 5,659 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View