Dr Jeyaleela 

If you have infected blood and need to identify the Plasmodium presence; you have lots of options but three important ones are

1. Using that infected blood made thick and thin slide and go for microscopy to identify the presence of Plasmodium and thin will help you in finding sub-species i.e Pf or PV using identification keys (characteristics)

2. You can also use Commercially available RDT kit. They will help in identifying the presence and absence of plasmodium infection and type of infection i.e. Pf or PF and even mix infection also

3. Most sophisticated is Using Molecular Diagnostic Tool i.e. Isolate DNA from Infected blood using commercial kit and go for diagnostic PCR for both plasmodium infection confirmation and sub-species identification. The best and pioneering reference for this purpose is G Snow et al.1997   

Hi,

If you want to isolate them from the infected blood, you may fancy the following Paper:

http://www.nature.com/nprot/journal/v6/n2/abs/nprot.2010.185.html

Thank you.

hi see this article

Some strains of Plasmodium falciparum, a human malaria parasite, evade the complement-like system of Anopheles gambiae mosquitoes.

Plasmodium falciparum lines differ in their ability to infect mosquitoes. The Anopheles gambiae L3-5 refractory (R) line melanizes most Plasmodium species, including the Brazilian P. falciparum 7G8 line, but it is highly susceptible to some African P. falciparum strains such as 3D7, NF54, and GB4. We investigated whether these lines differ in their ability to evade the mosquito immune system. Silencing key components of the mosquito complement-like system [thioester-containing protein 1 (TEP1), leucine-rich repeat protein 1, and Anopheles Plasmodium-responsive leucine-rich repeat protein 1] prevented melanization of 7G8 parasites, reverting the refractory phenotype. In contrast, it had no effect on the intensity of infection with NF54, suggesting that this line is able to evade TEP1-mediated lysis. When R females were coinfected with a line that is melanized (7G8) and a line that survives (3D7), the coinfection resulted in mixed infections with both live and encapsulated parasites on individual midguts. This finding shows that survival of individual parasites is parasite-specific and not systemic in nature, because parasites can evade TEP1-mediated lysis even when other parasites are melanized in the same midgut. When females from an extensive genetic cross between R and susceptible A. gambiae (G3) mosquitoes were infected with P. berghei, encapsulation was strongly correlated with the TEP1-R1 allele. However, P. falciparum 7G8 parasites were no longer encapsulated by females from this cross, indicating that the TEP1-R1 allele is not sufficient to melanize this line. Evasion of the A. gambiae immune system by P. falciparum may be the result of parasite adaptation to sympatric mosquito vectors and may be an important factor driving malaria transmission.

Falci-parum is derived from the Latin falx, meaning "sickle" and parum meaning "like or equal t. As the gametocytes of P. falciparum looks like sickle cell, it is called so. Collect blood for demonstration of MP few hours before paroxysm of fever or at least  12 hours after febrile paroxysm.This blood is  also good for antigen detection by dipstick test or for molecular study-PCR (DNA detection)