I am currently attempting to generate double knockout in mice which have the two genes on two different chromosomes. If I take male and female mice which are heterozygous for both the genes and mate them , I will get one in sixteen mice as either full knockout for both genes or complete wildtype. Since the aforementioned protocol forces me to breed several cages in an attempt to get full knockout and wild type as littermate controls , I was wondering if it is possible to compare full knockout of both genes with heterozygous littermate controls , which increases my chances of 1 in 4 mice being the ones I require (Assuming there is dosage compensation in the heterozygous ). Are there any publication which utilize the homozygous heterozygous comparison for double knockout mice. I found an online page " https://www.jax.org/news-and-insights/jax-blog/2011/december/double-your-knockouts-double-your-fun " which has outlined this idea but there are no citations in this article.
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