I am synthesizing a peptide on rink amide resin. So in one step I need to use Cs2CO3. I guess it can be removved by washing with water. Please help.
Caesium carbonate has good solubility in polar organic solvents so you can use DMF for washing.
In the water the resin shrinks and that is something you do not need.
I think you can wash by water then remove last traces of water by another polar solvent like alcohols, DMF or dioxan
Dear Kishore Mallela ,
In this condition water is not good option and it was not recommended, because water the resin shrinks and make some internal change so that, and amino acids have some sensitive nature even it's protected means.
Why can you go to water, even DMF or DCM will get remove Cs2CO3, if you are using water also, you need to remove traces of water by using some other solvents, it's make another problem. this much struggle not required for this.,
Hope you got.
Good Luck & Regards
R.Selva....
Dear Chemists
i tried the DMF , DCM as washing solvents but no use and the Cs2CO3 was found as a sediment in the libra tube . so i used 5%water in methanol and the Cs2CO3 is completely removed and sediment is not at all found after 3XSolvent washings with 5 minute agitation interval and traces of water is removed by the DMF washings.
And thank you all for the help and please let me know whether the solvent condition i used a good option or not..
Dear Kishore Mallela,
This because Cs2CO3 salt form so that it won't be dissolve you are right in this condition, if this is the end stage means you can use water no problem, but again you need to perform some other reaction or work on this means water not recommended.
in this condition no way, so you use water and then again wash with DMF to remove traces of water from the system and then you continue. no problem it's good option only.
Good Luck & Regards
R.Selva.........
O.K.
K. Mallela, you did good, and the resin recovers its property
yes the peptide is still intact , swollen the resin and it retained its property
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