Sodium azide is an inhibitor of ATPases, not just the ETC, so its effects are even more far reaching than just inhibiting mitochondria. This is why azide is extremely toxic and works so well as a preservative, it kills anything that requires ATP hydrolysis to live (just about everything).
Hi Dear,
No, this is not recommended to dissolve the drugs in PBS containing Sodium azide. As sodium azide is mitochondrial ETC chain inhibitor, so an obvious question will be there, about the effectivity of your drugs. If your cell's mitochondria (power house of cell) is under the question, you cant proceeds with that . Thanks
good luck!
Sodium azide is an inhibitor of ATPases, not just the ETC, so its effects are even more far reaching than just inhibiting mitochondria. This is why azide is extremely toxic and works so well as a preservative, it kills anything that requires ATP hydrolysis to live (just about everything).
You may consider providing more details of what you are trying to do. What would be the experiment following the drug treatment? If you are proceeding for flow cytometry, then you dont need sodium azide during drug treatment, assuming that drug does not need sodium azide to get solubilized. For flow cytometry you will need to add sodium azide for sure in one of the later important steps, again you need to mention how and what for you are processing the blood, and what is the experiments endpoint you are aiming for!
No, for the reasons stated by my colleagues above. If you are attempting to prevent microbial growth in your preparations so you can examine at a later date you can use antimicrobial cocktails as one would use in tissue culture. Keep in mind that Cox -2 inhibitors work when present so should you remove them secondary to your methods in cell preperation you are likely to obtain unreliable measurements. I suggest you conduct measurements on Cox-2 inhibition in a timely fashion.
Your question should be clear ed about your purpose.
People use sodium azide is as preservative for long time storage biological samples. It also protect from other microbiological infection. At the same time it toxic to cell and other organs like heart and brain. It also inhibit the immunological reaction in case of HRP conjugate. So better to use tissue culture grade antibiotic and anti fungal to avoid the contamination not sodium azide.
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