Hi Ricardo --
Though I haven't directly worked with this compound, my lab has previously administered antalarmin ICV using carboxymethylcellulose (0.5%) and DMSO. According to our records, for IP injections in mice, we made a suspension in deionized water with 0.5% carboxymethylcellulose (using a stir plate for up to 15 min). However, I don't think we used doses as high as 10 mg/kg. Feel free to email me if you have other questions!
Also see this link for an alternative (Funk et al., 2014).
RA
http://www.ncbi.nlm.nih.gov/pubmed/24944865
Ey Rachel! Thank you so much for the tip! I think centrally it can be administered more easily, even mixed with plain saline. IP seems to be more difficult, thanks a lot!
We have used DMSO to administer steroids via subcutaneous injections. It does cause acute pain at the injection site but we have seen no other adverse effects.
Dear Marcia H. Ratner
Did you administer the DMSO to human?(male female or children) would you please tell me how long the pain persisted?
I have read about pain induction in propofol which attributed to organic solvent.
Do you have any suggestion for reduction of pain induced by organic solvent (e.g. use of analgesics?)
We administered it to male Sprague Dawley rats.
For humans I would suggest only using DMSO as the vehicle if you are administering the drug via transdermal absorption with a patch rather than via injection.
We have tried using topical lidocaine to reduce pain at the injection site in rats but the effectiveness is limited.
If you do not mind my asking why do want to inject DMSO into humans?
Respected Mam (Marcia H. Ratner)
Can we use DMSO to administer testosterone subcutaneously in prepubertal rats ?
Is there any toxicity ?
We have not used it in prepubertal animals. You may want to minimize acute pain associated with administration by injecting it under isoflurane induced anesthesia to attenuate pain at site of injection and make sure not to exceed a 1 ml/kg dose of DMSO as a vehicle.
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