Dear Birhanu, in theory this is possible. You will need to check activity of vaccine after filtration to be certain that the filter does not absorb immunogenic components. I think also, you break the approved protocol for vaccine preparation. Therefore, there is concern of the safety. You will need to adjust this operation with regulatory authorities.

Dear Birhanu , i agree with Dmitry Kazansky, for the purification of rabies vaccine the gold standard is Zonal density gradient centrifugation, due to the cumbersome other chromatographic technique also available, but it should be optimize the binding efficiency, elution efficiency, and the immunogenicity , If you are using with 0.2 um millipore, may i know the membrane made up of and the it is only the microfiltration grade? or it need further ultrafiltration, on which buffer will use for the elution of rabies viral protein with that membrane?

Dear Birhanu

I suppose that your work is one step of development,( the answer of Dmitry is quite in regard with authorities), in phase R&D you can purify you harvest by ultracentrifugation and used some buffer to adequat titer on pellets and you can used filtration on 0,2µm to sterélise your product ( not mycoplasma), there are some technic of traitment of membrane before to pass the virus to evite the decrease of titer;

Good continuation