I want to take UV of metal complexes but complexes are partially soluble in solvents, i am using DMF and DMSO as a solvent. Can partially soluble complexes gives correct UV?
You can also use DRS UV in solid state and u should try sonication in order to make good suspension
It depends on what you consider a "correct" UV-vis spectrum. If you need an absorption coefficient, then complete solubility is necessary. You can also place a glass slide with a film of your material into the cuvette holder, but this is qualitative and also, in the solid state, the bands have normally slightly different positions than in the solution (and of course the spectra are solvent dependent). For DRS UV you would need an integrating sphere for quantification of the spectra of films and powders.
In addition what is being said above, If i am interested in UV absorption profile only then i will go ahead and filter the partially soluble solution through syringe filter and take the UV vis of solution.
Diffuse Reflectance IR and UV-vis Spectroscopy can be used in your case.
Find in attached a ppt. on: Diffuse Reflectance IR and UV-vis Spectroscopy
Modern Methods in Heterogeneous Catalysis
Friederike Jentoft
Some answers to a related question is in this link:
https://www.google.com.eg/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwjto-T1gIjPAhXSzRoKHc3wD6IQFggaMAA&url=https%3A%2F%2Fwww.researchgate.net%2Fpost%2FIs_there_any_difference_between_UV-VIS_and_UV-DRS_or_both_studies_are_same&usg=AFQjCNF-tF_yDazz9HXxi5UZBe3wOQPQIQ
You could work with solution 5% DMSO and 95% of the interest solvent. You could increase the concentration of DMSO until the solubilization, I think that not intefere the UV spectra.
One option is to get your material in solutions as much as possible, filter, and then to use a longer path length cuvette in order to increase the absorption profile. One "quick" way of doing this would be to use two separate cuvettes that are in series, as most instruments do not have the stage for a longer path length.
A preliminary question is: your compound is “partly” or “slightly” soluble? In the second case you can adopt the technic indicated in the Matthew answer while in the first case it should be possible that you have a mixture of two or more compounds, one of which is soluble while the other one(s) is/are not soluble in your condition. Consider this possibility.
if you can not dissolve the your compound by the increasing of DMSO percentage i.e. 10% or more, you can get UV spectra only to know the formation of complex.
You can study the UV-Vis spectrum of solid complex using diffuse-reflectance spectra.
you can used also as suspended in DMSO after sonication
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