I have an edible mushroom fruiting body and I am interested in finding out the compounds in the ethanol extract of the fruiting body. Many volatile organic compounds were reported in the mushroom.
Should I subject it to GCMS just to get a profile reading, and see what the major and minor peaks are? Whats are the pros and cons?
dear Mr. phan,
yes , you can run Gc-Ms for crude extract so that u can identify the known compounds present in it based on its rf value and its retention time.
Dear Phan,
If you wake your methanol extract in chloroform, ethyl acetate or acetone prior to running the GC-MS you will get better results. Certain agents/complexity in the crude will mask your peaks making your results hard to interpret. Also the life of your column(if it is important here) needs to be safeguarded; you want to run what you can flush out later and sustainably. Cheers!
Consider using a solid phase extraction cartridge to clean up your extract before injection.
In the past I have also had vastly different results from extraction plant materials with methanol of with hexane. Hexane is a lot more efficient for essential oils.
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