Dear Arif, Thank you for your reply. Actually I want to measure TiO2 particles suspended in water. Do I have to use same standard and wavelength you mentioned or different? 2ndly Do I have to measure Absorbance or transmittance ? Thank you !
I think yes you can. You need to first decide the wave length from 400 to 750 nm. This would be a unique wavelength for you and you wont be able to compare with other works! yet I'm pretty sure you can get reliable results. personally would prefer higher wavelengths within this range to reduce any contradiction. there can be impurities that can absorb lower wavelengths
Thank you Behzad, Absorption peak at different known concentrations is observed at 365 nm
Irfan, 395 nm is so close to UV and has a bit higher energy. you will see turbidity of a unique species at that range. Lets come to what you mean with turbidity. If you mean "number of particles" at a give volume, then you can use higher wavelength as well.
Previously I used UV Vis spectroscopy to measure iodide ions (I-) concentration in NaOH waste solution. Actually, now I have to measure the concentration (mass/volume) of TiO2 particles in waste water.
Turbidity measuring UV-VIS spectrophotometer is not a good idea. There will be scattering and constant values are difficult to obtain.
Concentration of particles in wastewater cannot be done by this, because high or highest contribution to absorbance will be due to other wastewater components. You may separate the particles by filtration or centrifugation. After ignition you may determine the mass. Maybe you can correct the values for others solids by using a blank of typical wastewater. Of course higher precision would be obtained if you would analyze content of Ti and then calculate TiO2.
Actually waste water is-------> Deionized water+ TiO2 powder (particles)
plz, may be some one send me the method of measure turbidity using UV/Vis Spectrophotometer at 660 nm
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